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Learning Goal: I’m working on a microbiology writing question and need an explanation and answer to help me learn.1. What material is used to solidify culture media and what is its source?2. What is the difference between a complex medium and a defined medium?3. What is the difference between selective media and differential media? Give an example of how each would be used?4. Given five different organisms with different oxygen requirements, describe the growth you would expect to see in a test tube culture of each.5. Describe two ways of growing anaerobic cultures, one chemical and one physical.6. Why are rickettsias and chlamydias not grown in laboratory cultures?7. What is a serial dilution? Why would one use a serial dilution?8. In a serial dilution where 1 ml of culture was plated from the 10-6 dilution, there were 253 colonies on the plate. What is the viable colony count of the organism?9. What is the difference between a pour plate and a spread plate for quantifying bacteria?10. Describe how each of the following would be used to measure bacterial growth: a. Plate countsb. Filtrationc. Turbidity using a spectrophotometerd. Direct microscopic count
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