Discussion This experimental exercise demonstrates basic laboratory practices necessary for handling and studying various micro-organisms. To prevent contamination of microbial cultures aseptic technique was employed. Lyompholized bacterial culture was reactivated and successfully sub-cultured into the broth and on agar through the application of the Aseptic technique. Aseptic techniques are critical in laboratory experiments and tests involving microorganisms to prevent contamination and ultimate interference of test results. To illustrate the subculturing and aseptic technique

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this experiment utilized Escherichia coli. The experiment aimed at keeping a pure culture (Escherichia coli) i.e. free from any present contaminants. Basic aseptic techniques include disinfection of the working area

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